Identification of Cis-regulator elements (CREs) of pollen-specific gene RMP1 and RMP2 in rice

Abstract

Tissue and organ-specific expression genes induced by Cis-regulator elements (CREs) are distributed in the promoter region of a gene. In rice, pollen-specific genes have been investigated to identify CREs related to specific expression in anther and pollen grain such as GTGANTG10, POLLEN1LELAT52... RMP1 and RMP2 genes that specifically express the early stage of pollen development were analysed their promoter region using NEW PLACE and PlantCARE tools. Results showed that 80 CREs were located in the RMP1 promoter and 95 CREs in the RMP2. Among them, 6 CREs are abundant distribution with from 12 to 25 copies, including ARR1AT, CAATBOX1, CACTFTPPCA1, DOFCOREZM, EBOXBNNAPA, and GATABOX. Interestingly, two pollen-specific CREs, GTGANTG10, POLLEN1LELAT52, were also found in RMP1 and RMP2 promoters. In which, GTGANTG10 was counted 14 copies in RMP1 and 21 copies in RMP2, whereas, POLLEN1LELAT52 was found 4 and 21 copies in RMP1 and RMP2, respectively.