Research on the one-step regeneration of sugarcane in KK3 variety (Saccharum officinarum L.) in vitro

Abstract

Abstract:
The research has successfully created the formula for direct regeneration medium of sugarcane variety KK3. In detail, the most suitable medium for direct regeneration from young leaf rolls of sugarcane variety KK3 was RE2 (MS + 5 mg/l α-NAA + 1 mg/l Kinetin), the regeneration rate was 25.27%, the number of shoots formed/1.0 g of young leaf rolls reached 11.56 shoots. The study showed that the position of the young leaf rolls cuttings suitable for direct regeneration was the cuttings located from 2-6 cm away from the growth apex. The shoot regeneration rate reached 25.40%, the regenerating samples were 25.40%, good yield, and a high rate of multiple shoot formation. When using plant cuttings with a thickness of 1 cm, the highest regeneration rate was 25.58%, and the ability to form multiple shoots was high. Pre-cultivation time had a positive effect on the ability to regenerate shoots directly from young leaf coils of sugarcane variety KK3. When conducting pre-cultivation in four days will help increase the regeneration ability of the sugarcane variety, reaching 47.49%. MS medium + 5 mg/l α-NAA + 1 mg/l Kinetin supplemented with 30% coconut water showed the highest regeneration ability, reaching 52.95%, number of shoots reaching 52.33 shoots/1 g of young leaf rolls.