Creating in vitro initial material sources of Vanilla planifolia Andr.

Abstract

There are many methods to create in vitro initial material. However, in this study, we used a variety explant of Vanilla as research material to find the appropriate tissue type and medium for initial materials and the optimal disinfection method. The results showed that the sample which was sterilized with 0.1% HgCl₂ for 9 minutes expressed the best effect. The culture medium supplemented with 3 mg/l BAP gave the best shoot regeneration in both shoot apex and axillary bud with 2.13 shoots/samples and 2.50 shoots/samples, respectively. The root tip is the most suitable type of tissue for callus formation on the MS medium with 3 mg/l 2,4-D with the highest percentage of callus tissue regeneration (60%). The medium supplemented with 4 mg/l BAP, 0.5 mg/l IBA, and 0.5 mg/l kinetin most suited for the differentiation of callus cells into PLBs with a PLB-induced callus ratio of 33.33%, and the PLB grew well.