Application of nested PCR to detect Ancylostomaspp. and Necator americanus infections in human

Abstract

In the present study, a nested PCR based approach was applied for the specific detection of Necator americanus and Ancylostoma spp. in human faeces in Duc Lap Thuong commune, Duc Hoa district, Long An province. A total of 80 DNA larva samples were extracted from 80 positive human faecal samples using the Sasa method. The results of the nested PCR based method amplifying a fragment of the 28S rRNA-ITS2 region exhibited that, the infection with Necator americanus was found in 66 (82.5%) samples, the infection with Ancylostoma spp. was found in 5 (6.25%) samples, and 9 (11.25%) samples represented the co-infection with both species. The results showed that the present PCR approach is a valuable complementary tool for the diagnosis of Necator americanus and Ancylostoma spp. infections in human and for monitoring the parasitic infection control programs in Vietnamese provinces and cities.