Mycelial growth and fruting body formation of the king oyster mushroom Pleurotus eryngii (DC.: Fr.) strain E2

Abstract

The aim of this study is to evaluate the influence of some factors on mycelial growth and fruting body formation of the king oyster mushroom strain E2. Based on obtained results, the strain E2 is able to grow well on the modified PGA (Potato, Glucose, Agar) medium in a wide pH range from 5 to 12. The optimal pure culture medium for mycelial growth is the PGA supplemented with yeast extract. Among of 5 mother spawn media, MT1 (99% grain of rice + 1% CaCO3 ) could be considered as the best medium with growth rate of 6.14 mm/day. The strain E2 is capable of growing to form and develop primordia in all five substrate mixtures. In addition, GT3 (59% sawdust + 20% corn cob + 20% wheat bran + 1% CaCO3 ), GT2 (79% corn cob + 20% wheat bran + 1% CaCO3 ), and GT1 (79% sawdust + 20% wheat bran + 1% CaCO3 ) showed a high biological efficiency with 54.37%, 51.84%, and 48.85%, respectively.