Study the ability to induce the adventitious root of Paramignya trimera in vivo hydroponic conditions

Abstract

Xao tam phan, Paramignya trimera, is a precious medicinal plant belonging to genus Citrus (Rutaceae family), mainly distributed in Khanh Hoa and Tay Ninh provinces, Vietnam. For many years, Paramignya trimera has been used as an effectively medicinal plant to treat cancer, especially for liver cancer. Because of the natural active compounds of Paramignya trimera distributed mainly in the roots, the over-exploitation of Paramignya trimera for roots over time has resulted in the serious decline of this species. In nature, the growth of Paramignya trimera is slow, so it takes many years to be exploited. Therefore, in addition to planting Paramignya trimera in natural conditions to harvest roots, an altinative method should be developed to actively produce large amounts of roots for pharmaceutical industry. The study aims to create an method for the stimulation of adventitious roots of Paramignya trimera under hydroponic conditions. The results showed that Paramignya trimera was able to grow under hydroponic environment (WPM/6 medium) at the survival rate of 96.21%. The formation of adventitious roots in Paramignya trimera was observed in the WPM/6 medium supplemented with IBA (7.0 mg/l) and α-NAA (7.0 mg/l). After 8 weeks, the number of newly formed roots was 21.12 roots per plantlet, and the rate of root formation reached 90.6% in all cultured plantlets. The results exhibited that the Paramignya trimera could survive, grow, and develop well under hydroponic conditions.