Changes in DNA sequence and fiber protein structure of HAdV-3 causing conjunctivitis in Vietnam

Abstract

Protein fiber is important for the infection of HAdVs. However, in Vietnam, there are no thorough researches on mutations on genes or protein structures of either HAdVs or HAdV-3 causing conjunctivitis. In this research, the authors sequenced the coding sequence of fiber protein of HAdV-3 isolated in Vietnam and used bioinformatics software to analyse amino acid changes. The results exhibited fifteen substitutions in DNA sequence, ten of which led to amino acid substitutions. Four of ten substitutions in protein fiber sequence (Q150E, S207L, H246D, M272T) potentially affected the interaction between this protein and CD46 receptor of human cells.