The method to identify the genetic frequency of ridgeback mutation on Phu Quoc ridgeback dog (Canis familiaris) in Vietnam

Abstract

Phu Quoc dog is one of typical species of Phu Quoc Island, Vietnam. The distinguishing feature of this dog is the ridgeback on the spine. The ridge mutation is an autosomal dominant trait associated with dermoid sinus symptom which causes degeneration and puppies death. The dominant allele causing the ridge is denoted as R whereas the recessive wild type-allele is denoted as r. This means that a ridged dog may either be homozygous (R/R) or heterozygous (R/r) for the Ridge allele whereas all ridgeless dogs should be homozygous r/r for the normal allele. In this study, real-time PCR with 4 primer pairs was used to evaluate the copy number variation (CNV) of the 133 kb region on chromosome 18 to identify the genetic frequency for this dominant mutiation. The results could be used to test the genotype of dominant mutiation for conservation programs, restrict the development of dermoid sinus symptom, an make balance in population growth of this endemic species.