Determination of polysaccharide and phenolic contents and antioxidant activity of the crude extract from mycelial biomass of strain Tropicoporus linteus NTH-PL3

Abstract

Tropicoporus (synonym Phellinus) mushroom is one of the precious medicinal mushrooms and has been proven to play a significant role in supporting health. However, studies on the Tropicoporus mushroom have only been carried out in our country within the last ten years, so the scientific data on this mushroom source is not really rich. This study aimed to add research data on molecular identification of strain NTH-PL3 collected in nature and determination of bioactive compounds, as well as antioxidant activity of the total extract from mycelium of Tropicoporus. Results of rDNA ITS sequence analysis showed that strain NTH-PL3 belongs to Tropicoporus linteus species with the highest similarity in ITS sequence of 99.87%. The total yield of bioactive compounds extracted from the mycelium using 75% methanol was 29.75±3.84%. In the crude bioactive extract, total polysaccharide content was 23.72±1.34 mg glucose/g and 18.54±1.87 mg gallic acid/g for phenolic. EC₅₀ values for 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferrous ion chelating ability of total crude bioactive extract from mycelium of Tropicoporuswere 268.54±3.89 and 411.65±10.87 µg/ml, respectively.