Induced breeding of Sahyadria denisonii using different hormonal substances

Abstract

Research on spawning stimulation of Sahyadria denisonii was carried out from June 2022 to October 2022 at the Research and Development Center for High-Tech Agriculture with three inducing agents, HCG, Ovaprim, and LHRHa + Domperidone. Each concentration of a hormone used in the experiment was applied on 5 pairs of brood fish. A 1 ml syringe was used for injecting to the anterior region of the dorsal fin by using a single-injection method and the injection timing of fish was 18 hours. Results showed that Ovaprim had an effect on inducing ovulation and laying eggs at a dose of 0.4-0.5 ml/kg of female fish. Female fish were exposed to hormones and ovulated after 11 hours 25 minutes to 11 hours 28 minutes from the time of injecting, 80% reproduction rate, actual fecundity from 747.67 to 750.33 eggs/female, 84% fertilisation rate, 84% hatching rate, time of embryo development was 30 hours at a water temperature of 29^oC. The average length of new-hatched larvae was 3.2 mm with a large amount of yolk sac, the time to consume the yolk was from 3 to 4 days. HCG and LHRHa + Domperidonedid not have any effect on ovulation and spawning in fish.