Application of the SDS-PAGE method to study the effect of SDS content on protein extraction in rice

Authors

  • Nguyen Thi Dong*
  • Tran Trung
  • Vu Thi Thu Ha
  • Tran Dinh Phong
  • Nguyen Thi Quynh Hoa

Keywords:

Protein extraction, rice protein, SDS, SDS-PAGE

Abstract

This article presents how sodium dodecyl sulfate (SDS) affects the ability to
extract proteins in rice. Analysis data of polyacrylamide gel electrophoresis
spectrum available sodium sulfate dedocyl (SDS-PAGE), UV-Vis spectrum,
and Raman spetrum showed the SDS concentration in the rice protein
extraction solution (0.05 M tris, pH8, 5 M urea, 5% 2-ME) affected the
ability of rice protein extraction. The ability to extract the highest rice
protein content was reached at the SDS concentration of 3%.

Classification number

1.4

Author Biographies

Nguyen Thi Dong

Hung Yen University of Technical Education

Tran Trung

Hung Yen University of Technical Education

Vu Thi Thu Ha

Institute of Chemistry, Academy of Science and Technology of Vietnam

Tran Dinh Phong

Hanoi University of Science and Technology

Nguyen Thi Quynh Hoa

Hung Yen University of Technical Education

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Published

2018-01-25

Ngày nhận bài 8/9/2017; ngày chuyển phản biện 12/9/2017; ngày nhận phản biện 10/10/2017; ngày chấp nhận đăng 1/11/2017

How to Cite

Dong, N. T., Trung, T., Ha, V. T. T., Phong, T. Dinh, & Hoa, N. T. Q. (2018). Application of the SDS-PAGE method to study the effect of SDS content on protein extraction in rice. Version B of Vietnam Journal of Science and Technology, 60(1). Retrieved from https://b.vjst.vn/index.php/ban_b/article/view/259

Issue

Vol. 60 No. 1 (2018): Volume 60 - Number 1 - January 2018

Section

Natural Sciences