In vitro propagation of Philodendron hederaceum “Lemon Lime”

Abstract

This study aims to examine the in vitro propagation of Philodendron hederaceum “Lemon Lime”. The results showed that MS medium supplemented with 1.0 mg/l BA was suitable for the rapid multiplication stage, with the rate of shoot proliferation being 2.73 times, the shoots being 5.38 cm in height and the number of leaves/shoot was 4.33 after 6 weeks of culture. In the next stage, in vitro shoots were transferred to MS medium containing 0.25 mg/l IBA for rooting. Within five weeks of culture, 100% of shoots induced roots with a mean of 3.9 roots/plant and the root length was 11.04 cm. In the acclimatisation phase, plantlets achieved 100% ex vitro survival, increased by 2.19 cm in height and 1.96 leaves after four-week acclimatisation in a substrate composed of peatmoss:soil:coconut husk (1:1:1). These research results could be used to cultivate this plant with high proliferation rate and good commercial quality providing seedlings for the floriculture market in Vietnam.