Identification of genetic relationships of Acacia auriculiformis clones using ISSR markers

Abstract

In this study, seven clones of Acacia auriculiformis, collected in Cam Lo district, Quang Binh province were assessed for genetic diversity using 15 ISSR markers. The results showed that seven Acacia clones had an average level of genetic variation. In which, four ISSR markers including ISSR1, ISSR3, ISSR8, and ISSR15 showed higher polymorphism information content (PIC) value than the others. The results analysis indicated that 30 out of 40 DNA fragments were polymorphic (75%, PIC=0.28-0.33). The ISSR8 exhibited the highest genetic diversity index (0.33), while ISSR15 showed the lowest diversity (0.28). Seven clones of A. auriculiformis were divided into two main groups: group I comprised four
clones: Clt7, Clt18, Clt19, and Clt26; group II included the remaining three clones: Clt25, Clt43, and Clt57. Genetic variation was correlated with plant growth ability. This result exhibited that the ISSR marker was an efficient tool in assessing and selecting the good genetic resource of A. auriculiformis for the forestry tree breeding program in Vietnam.