Evaluate the ability of nitrogen fixation and Indole-3-acetic acid (IAA) biosynthesis of Flavobacterium anhuiense MN47 isolated from tea-growing soil in Thai Nguyen

Abstract

The goal of this study is to select potential bacterial strains capable of fixing nitrogen and Indole-3-acetic acid (IAA) biosynthesis. From 36 tea-growing soil samples collected in La Bang commune, Dai Tu district, Thai Nguyen province, the strain MN47 was selected, with the highest ability to fix nitrogen and biosynthesise IAA of 20.41 and 106.08 μg/ml, respectively. pH 7.5 and at 30^oC are appropriate conditions for strain MN47 to
synthesize IAA in Ashby medium supplemented with 0.1% tryptophan. The results of comparing the 16S rRNA gene sequence of strain MN47 with species published on EzTaxon showed that strain MN47 has the highest similarity (99.86%) with Flavobacterium anhuiense D3^T. Based on 16S rRNA gene sequence analysis data, strain MN47 belongs to the genus Flavobacterium, with the scientific name F. anhuiense. This is the first publication
on the ability to fix nitrogen and biosynthesise IAA of F. anhuiense MN47, which is a potential source of strains for the development of microbial products for agricultural applications.