Activation culture and proliferation of γδT cells from lung cancer patients

Abstract

Autologous γδT lymphocyte-based immunotherapy is currently an efficacious and safe treatment method for various types of cancer, including lung cancer. The study was conducted on 10 lung cancer patients, and the γδT cells were isolated, activated, proliferated, and evaluated from 10 ml of blood of each patient. The number of γδT cells isolated from 10 ml peripheral blood was 1.96x104 cells. The number of cells after 14-day culture was 2.93x108, and the cell survival rate was 90%. 83.2% of the cultured lymphocytes was γδT cells. The cultured γδT cells exhibited immune activities, such as cytotoxic activity and increased cytokine secretion.