Distribution of Candida species collected from blood of Vietnamese patients based on pcr-rflp kit

Abstract

The purpose of this study was to identify yeast species isolated from the blood samples by PCR-RFLP kit. In this study, the authors used PCR-RFLP kit as a method for the specification of yeast isolates from 93 blood samples. The amplified product was digested using restriction enzyme by RFLP to identify candida species. The results showed that, the most frequently found species was C. tropicalis (n = 47, 50.54%), and followed by Candida albicans (n = 18, 19.35%), C. parapsilosis (n = 16, 17.20%), C. glabrata (n = 6, 6.45%), C. krusei (n = 1, 1.08%) and 5 Candida spp. By sequencing the PCR products, these 5 isolates were identified as C. mesorugosa. In conclusion, PCR-RFLP is an easy, highly sensitive, specific and valuable tool for the rapid identification of yeast isolates from blood. This rapid method will help clinicians to decide on empirical therapy in yeast cases before antifungal susceptibility results are available.