Developing a procedure for isolation of some vina-ginsenosides to be used as reference standards in standardization and quality control of Vietnamese ginseng

Abstract

Vina-ginsenosides are characteristic minor saponins in Vietnamese ginseng (Panax vietnamensis Ha et Grushv.- Araliaceae). The study aimed to develop a procedure for isolation of some vina-ginsenosides to be used as reference standards in standardization and quality control of Vietnamese ginseng. The study includes the extractions and isolation of 3 vina-ginsenosides which share the same structure of ocotillol and the verification of these saponins with various criteria including TLC purity, HPLC quantitative analysis, MS and NMR spectra. The isolated saponins were identified as vina-ginsenoside R2, -R10, R11 with the HPLC purity > 95%. By using the preparative reverse-phased HPLC system with an ODS column, the isolation procedure of these compounds could be simpler and faster. The result showed the developed method was suitable for isolation of these compounds to be used as the reference standards for standardization and quality control of Vietnamese ginseng.