Comparison of extraction process from brown seaweed Sargassum by different methods and evaluation of antioxidant possibility by DPPH method

Abstract

The study was conducted on two types of brown seaweed Sargassum feldmannii and Sargassum polycystum which were collected from the sea of Khanh Hoa and Ninh Thuan provinces. Acquisition of extraction from the samples was operated by three methods: Soxhlet, ultrasound method and method using enzyme Viscozyme L. Results of ultrasound method showed that the extract recovery was 7.095±0.055% in S. feldmannii and 8.373±0.122% in S. polycystum; their free radical scavenging activities (DPPH) were 63.037% and 62.720% respectively. For methods using enzymes Viscozyme L, the extract recovery was 7.192±0.213% in S. feldmannii and 8.020±0.186% in S. polycystum; their free radical scavenging activities (DPPH) were 61.642% and 62.766% respectively. The effective extraction of these two methods was higher than that of the Soxhlet method  which the extract recovery was 6.774±0.060% in S. feldmannii and 6.575±0.413% in S. polycystum; their free radical scavenging activities (DPPH) were 54.301% and 56.503% respectively, compared to the reference substances as vitamin C at the same concentration.