Optimisation of medium composition for recombinant antibacterial peptide production from Bacillus subtillis DB104 by using response surface methodology

Abstract

Antimicrobial peptides (AMPs) were described as having a broad spectrum of antibacter H2PO₄ , and molasses were considered significantly (p<0.05). These factors were subsequently optimised using the response surface methodology (RSM). The obtained results showed the appropriate medium for the biosynthesis of peptides included 12.4 g/l pepton, 5.1 g/l K₂HPO₄ , and 8 g/l molasses. After 36 hours of fermentation, the peptide activity reached the highest level of 1,600 AU/ml, 2.5 times higher than that of the pre-optimized one (640 AU/ml).