The effectiveness of Blumea lacera extract on chronic renal failure animal model induced by adenine

Abstract

Objective: to evaluate the effectiveness of Blumea lacera extract on chronic renal failure model induced by adenine in mice. Methods: Blumea lacera was extracted by 96% ethanol three times, then the liquid was evaporated with low pressure to get EtOH extract. Swiss mice were induced with renal failure by adenine oral administration (100 mg/kg) every 2 days. After 35 days, 16 renal failure mice were randomly divided into two groups: (i) HKT group, Blumea lacera EtOH extract was orally administrated (at the dose equivalent to 8 g/kg) every 2 days; in other days adenine was orally administrated (100 mg/kg); (ii) ST group: adenine was orally administrated (100 mg/kg) every 2 days, without Blumea lacera EtOH extract. Experimental periods prolonged to 21 days. Bioparameters were collected such as blood cell count, urea, creatine, renal histopathology. Results: at the end of 21- day duration, the similarity between the control group and the HKT group was identified. ST group showed the significantly higher urea and creatine concentrations than other groups; red blood cells and hemoglobine were significantly lower than those in other groups. The renal structure image and superstructure image showed that, there was no renal damage in the HKT group, while the damage was observed in mitochondrial level in the ST group. Conclusion: Blumea lacera EtOH extract showed the effectiveness on renal failure animal model induced by adenine