Isolation of taxoids in 100% methanol extract from the needles of Taxus wallichiana Zucc. cultivated in Lam Dong

Abstract

The aim of this study was to isolate taxoids in 100% methanol extract from the needles of Taxus wallichiana Zucc. cultivated in Lam Dong province by column chromatography with usual solvents. From 75 g of 100% methanol extract, the isolated taxoids were identified as taxinine B (425 mg), taxuspine F (732 mg), taxchinin B (703 mg), and taxuspine D (83 mg). The chemical structure of taxoids was elucidated by means of UV-Vis, IR, MS, NMR spectroscopies and comparison with reference data.