Genetic diversity of matK gene in Vietnam’s longan germplasm

Abstract

The genetic diversity survey on the matK gene segment of 829 nucleotides among 31 Vietnam’s longan samples has identified tranversition mutation (T>G) at the 939 downstream position of the sequences in 11 longan samples, which might be the molecular identification to distinguish Vietnam longan varieties with others. These matK nucleotide sequences have been registered with NCBI codes (KR073235- Ban Nguyen, KR073239- Long Gia San, KR073240- Tieu Vung Tau, KR073241- Tieu Da Me, KR073243- Nhan Sai Gon, KR073245- Com Vang Banh Xe, KR073249- Xuong Com Rao, KR073251- Long Tieu, KR073252- Xuong Com Vang Ba Ria, KR073253- Xuong Com Trang and KR073255- Nhan Vung Tau). The phylogenetic tree analysed by the Neighbour Joining method based on the 829 nucleotides of matK gene has exactly grouped all surveyed sequences. In addition, this analysis has also clearly separated the Dimocarpus and the Litchi, Arytera, Sapindoidaea, and Cupaniops is in Sapindaceae. Eleven longan sequences (N10, N14, N16, N17, N19, N22, N26, N28, N29, N30, N32) have been distinguished with the Vietnam’s longan and other reference sequences.