Liquid fermentation technology, its applications in cultivation of golden oyster mushroom (Pleurotus citrinopileatus)

Abstract

Due to containing several polysaccharides, essential amino acids, golden oyster mushroom (Pleurotus citrinopileatus) has high nutritional values as well as medical values. The aim of this study is to investigate optimal culture conditions for mycelium growth under liquid fermentation and using spawn produced from liquid fermentation in cultivation of golden oyster mushroom. The experiment results showed that the optimal culture conditions for mycelial biomass was 20 g/l fructose + 5 g/l pepton + 1.0 g/l KH2PO4 + 0.5 g/l MgSO4.7H2O; pH = 7.0 for 96 hours with the oxygen mode 0.7 V/V/M. The use of liquid spawn for mushroom cultivation can reduce the spawn running (4-5 days), the percentage of contamination (1.5%), and the fruit body formation time (3-4 days) as compared to the traditionally used grain spawn.