The evaluation on the polymorphism of cGH and cGHR genes in the Mong Tien Phong chickens

Abstract

The chicken growth hormone (cGH) gene and the chicken growth hormone receptor (cGHR) gene play a crucial role in production the traits of chicken. The objective of the research is to identify the single nucleptide polymorphisms (SNPs) at the positions: G1705A in cGH-int3, G3037T in cGH-int4, and 561 in cGHR-int2 (amplified- PCR product) in Mong Tien Phong chicken populations by the PCR-RFLP technique and gene sequencing. The research results indicated that the polymorphism at the locus cGH-int4 was highest, and the genotype frequencies of GG, TG, and TT were observed as 0.361, 0.213, and 0.426, respectively. Consequently, cGH-int4/ Bsh1236I/G and T allele frequencies were 0.468 and 0.532, respectively. The polymorphism at the locus cGH-int3 was found lower with the cGH-int3/EcoRV/G allele frequency of 0.92 and the cGH-int3/EcoRV/A allele frequency of 0.08. Whereas, no polymorphism was detected in the locus cGHR-int2 since all tested chickens carried cGHR-int2/HindIII+/+ genotype.