Genetic diversity of Cinnamomum samples in Thanh Hoa based on RAPD markers

Abstract

The classification of Cinnamomum species is still based on morphological characters which are influenced by environmental factors. Therefore, molecular markers such as randomly amplified polymorphic DNA (RAPD) were employed in order to find a more reliable approach for identifying Cinnamomum samples correctly. RAPD-PCR analysis involving the 22 random primers was used to assess the genetic diversity of 32 Cinnamomum sp. samples that have been originated from Thanh Hoa province. The results showed that a total of 3137 bands were detected, with 126 type bands at different sizes. Out of which, 61 polymorphic bands (48.41%) and 65 monomorphic bands (51.59%) were amplified. The number of polymorphic bands detected by each RAPD marker ranged from 3 to 11, with a mean of 5.8 bands per locus. Genetic similarity coefficients of 32 Cinnamomum samples ranged from 0.67 to 0.97. Based on genetic similarity coefficient, 32 Cinnamomum samples were divided into six major groups. Identification of 2 unique bands observed in 2 primers that can be used to identify exactly 2 different genetic resources. The OPN20 and UBC728 primers can be used to identify TT02 and XC24 varietiy, respectively. The obtained results are useful for classification and exact identification of some genetic resources that will be provided for the conservation and breeding.