A study into stroma cultivation medium of Cordyceps militaris

Abstract

In this study, Cordyceps militaris strain CM3 stored in the collection of Research and Development Center for High Technology Agriculture was used. 9 media for C. militaris cultivation were investigated, and the optimal cultivation medium was found as M9 which consisted of the following components: brown rice 60 g, silkworm pupae 10 g, CaCl₂ 0.1 g/l, folic acid 5 ^g/l, distilled water 90 ml; this medium is suitable for stroma production of C. militaris. Fresh weight, dry weight, and the number of stroma reached 19.54 g/bottle, 3.8404 g/bottle, 70.3 stroma/bottle, respectively. Biologically active substance contents of Cordyceps militaris stroma are adenosine (2.0027 mg/g), cordycepin (7.7551 mg/g), polysaccharide (166.20 mg/g), ergosterol (1.7369 mg/g).