Study on the rapid multiplication and planlets regeneration of (Vanilla planifolia Andr.

Abstract

This study was worked out to determine the protocol for rapid multiplication and planlet regeneration of Vanilla planifolia. The explants were cultured on the Murashige and Skoog (MS) basal medium containing 3% sucrose and 0.65% agar supplemented with coconut water and different plant growth regulators, either alone or in combination. The results indicated that the MS medium supplemented with 1 mg/l BAP, 0.1 mg/l IBA and 10% coconut water was most suitable for shoot multiplication with 5.4 shoots/explant, the average shoot height of 2.03 cm, the average leaf number of 3.67. Our experiments also showed that shoots were rooted on the MS medium with 1 mg/l NAA had the highest rooting percentage of 96.67%, and the highest number of roots was 3.13 roots/ plantlet.