Micropropagation of (Dendrobium loddigesii Rolfe by thin cell layer culture

Abstract

With the aim at preserving and developing highly precious Dendrobium loddigesii Rolfe, a study on its multiplication was carried out, in which a traversed thin cell layer (tTCL) excised from in vitro shoot tips was cultured in different media. Results showed that highest number of protocorm-like bodies (PBLs)/tTCL produced was obtained on the VW medium supplemented with 20 g/l sucrose + 10% coconut water + 7 g/l agar + 1.5 mg/l BA (the obtained number was 30.1 PLBs/tTCL at 6 weeks after culture) whereas the highest quantity of generated shoots was recorded in the VW medium added by 20 g/l sucrose + 10% coconut water + 7 g/l agar + 1 g/l activated carbon + 2 g/l peptone  + 1.5 mg/l BA + 0.5 mg/l IBA + 30 g/l mashed pumpkin + 1 g/l Spirulina algae (16.82 shoots/explant) at 8 weeks after culture. In addition, the VW medium supplemented with 20 g/l sucrose + 10% coconut water + 7 g/l agar + 1 g/l activated carbon + 1.0 mg/l IBA was considered to be most suitable for root development and growth of in vitro plants (7.3 healthy roots/plant after 6 weeks).