Building a procedure for testing level of sperm DNA fragmentation

Abstract

DNA fragmentation is known as a common reason contributing a noticeable proportion in infertility treatment, approximately 10% of patients who have normal semen parameters possesses a high level of DNA fragmentation which significantly reduces infertility treatment effect. The most common method of testing is counting number of sperm processed by Halotech Kit and then setting up DNA fragmentation index (DFI) to evaluate and anticipate useful assisted reproductive technology. This research worked on building up a suitable process of timing and required chemical solution formula from raw separated chemicals to reduce financial cost compared with the commercial Kit. Samples were handled parallel by this working process and the given DNA Halotech Kit to compare and evaluate reliability. Results exhibited that this method brought up a processing effect in similar pattern with the commercial Kit.