Isolation, identification and characterisation of phosphate solubilising bacteria from rice rhizosphere

Abstract

This study was carried out with the aim to isolate and characterise the phosphate solubilising bacteria from rice rhizosphere soil samples in Gia Loc district, Hai Duong province. From soil samples collected from the area, 14 bacterial strains with phosphate solubilising activity were isolated. Two strains of them GL2 and HD3 exhibited the maximum activity and could produce phytohormone IAA and siderophore. The strains GL2 and HD3 exhibited highest phosphate solubilising activity when they were cultured in a NBRIP medium with glucose as carbon sources and yeast extract or salts (NH4)2SO4, NH4H2PO4, NH4NO3 as nitrogen sources at 30ºC, pH 5-7. The bacterial strain HD3 had identified and named Pseudomonas aeruginosa HD3.