Verifying the accuracy of self-manufactured test kit for determination of fructose concentration in semen for male infertility diagnosis

Abstract

Fructose is formed in the seminal vesicle under the action of testosterone and is secreted along with the vas deferens during each ejaculation, so fructose is considered a biochemical agent that faithfully reflects the function of these components. Fructose concentrations in normal semen confirm the role of testosterone and the function of normal seminal vesicles, without the phenomenon of obstruction. Materials and methods: the semen samples were obtained from 30 male partners of infertile couples who had consultation at Genetic Counseling Centrer of Hanoi Medical University Hospital, and the semen samples were analysed for the seminogram parameters. Fructose concentration was assessed using self-manufactured test kit (according to the improved ROE method). Results showed that the optimal solution concentration of TCA was 10%; composite color composition included 2.5 ml of HCl 30% and 0.25 ml of resorcinol 0.1%; repeatability was CV%=1.407% (<5%); intermediate precision was CV%=2.032% (<5%); correctness was tex=0.906<tt =2.262. In conclusion, the manufacturing procedure of the test kit for determination of fructose concentration in seminal fluid was successfully verified