Study on in vitro propagation of (Curculigo orchioides Gaertn. through apical meristem culture

Abstract

In this study, authors propagated Curculigo orchioides Gaertn plants using the apical meristem culture method. The results indicated that: the most appropriate medium for multiplication of shoots was the MS medium supplemented with 30 g/1 sucrose + 5.5 g/1 agar + 200 ml/l coconut water + 1 g/l activated charcoal + 1.5 mg/l TDZ + 0.5 mg/l IBA + 1.0 mg/l AgNO3 + 50 mg/l Spirulina algae, with the results of 20.8 shoots/ explant and 5.2 leaves/plantlet after 6 weeks of culture. Root formation of shoots carried out on the MS medium supplemented with 30 g/1 sucrose + 5.5 g/1 agar + 200 ml/l coconut water + 1 g/l activated charcoal + 0.5 mg/l IBA gave the best result. In nursery, a mixture of humus + coconut fiber powder (70:30 ratio) was regarded as the best subtrate due to the high survival rate of plantlets (98%) and healthy plantlets (16.60 cm high with 6.9 leaves and 6.3 new roots/a plantlet) at 10 weeks after planting.