Efficiency of inhibiting infectious bursal disease virus in vivo

Abstract

A study on efficiency of recombinant chicken interferon (rChIFN) in treatment of Gumboro disease by using only chicken interferton alpha (ChIFN-α) or combination with interferon gamma (ChIFN-γ) was carried out in 3-week-old chickens. The experiment was performed as procedure: firstly, chickens were exposed to eye/nasal drops with virulent Gumboro disease virus with dose of 1x105 ELD50 per chick. After 8 hours challenges virus, chickens were exposed to eye/nasal drops with rChIFN-α for one of six experimental groups. The chickens were used rChIFN-α 100 µg /chick having the protection and the survival rate in respectively 56.67% and 93.33%. The chickens were used rChIFN-α 100 µg/chick combination rChIFN-γ (1 µg/chick) having the protection and the survival rate in respectively 70.00% and 93.33%. The chickens were used rChIFN-α 100 µg/chick having the protection and the survival rate in respectively 36.67% and 80.00%. The chickens were used rChIFN-α 100 µg/chick combination rChIFN-γ (1 µg/chick) having the protection and the survival rate in respectively 53.33% and 86.67%. Meanwhile, the positive control group (chickens were infection, untreated), chickens were not protected (100% infection rate) and survival rate was only 60.00%; Negative control group (non-viral chickens, not treated with rChIFN) whole chickens were not infected and 100% survival rate. The results suggested that using rChIFN-α increased the protection and survival rate of infected chickens Gumboro depends on rChIFN concentration. Concurrently, using rChIFN-α combine with rChIFN-γ had increased treatment efficiency compared to using only rChIFN-α.