The effects of culture medium on in vitro oocyte maturation and embryo development of high yield cow

Abstract

The purpose of this study is to assess the influences of maturation and embryo culture media on the production of in vitro bovine embryos. Oocytes used in this study have been collected from live cattle by ultrasound method, in which the A, B oocytes have been estimated about 7.2 oocytes per ovary. Percentage of matured oocytes, cleavage, morula, blastocyst and hatched embryos produced from oocytes cultured in maturation medium including TCM 199 + 10% FCS + 5 μg/ ml FSH have been 89.31, 90.14, 47.65, 23.44 and 3.9% respectively, which are significantly higher than those produced from oocytes in TCM 199 + 20% FCS or Ham’s F10 + 10% FCS + 5 μg/ml FSH or Ham^,s F10 + 20% FCS (p<0.05). The combination of oocytes matured in TCM 199 + 10% FCS + 5 μg/ml FSH and embyros cultured in SOFaa + 5% FCS has resulted in more effective production of in vitro bovine embryos than embryos cultured in CR1aa + 5% FCS. There was no difference in the proportion of cleavage between oocytes cultured in SOFaa (90%) and in CR1aa (90.14%). However, the rates of morula, blastocyst, and hatched embryos produced in SOFaa medium (59.72, 35.42 and 15.27% respectively) have been significantly higher than those produced in CR1aa medium (47.65, 23.44 and 3.9% respectively). This study has demontrated that using TCM 199 + 10% FCS + 5 μg/ml FSH for oocyte maturation combined with SOFaa medium supplemented with 5% FCS for embryo culture could get more in vitro bovine embryos.