Effects of plant growth regulators on the morphogenesis of Codonopsis javanica (Blume) under in vitro conditions

Abstract

Codonopsis javanica (Blume) belongs to the Campanulaceae family and is an important medicinal plant. However, the number of this plant in the nature has been decreasing dramatically because of its narrow distribution, overexploitation and low germination of seeds. The results of studying the effects of plant growth regulators on the formation of shoots and roots of this species under in vitro conditions showed that: the explants sterilized with 3% NaOCl for 10 min and then cultured in SH medium supplemented with 3% saccharose + 0.8% agar + 10% coconut water (CW) yielded the highest survival and clean explants (66.5%) after 2 weeks of culture. After 6 weeks, the capability of in vitro shoot multiplication was the highest (19.28 shoots/explant, length of 4.67 cm/shoot) in SH medium supplemented with 3% saccharose + 0.8% agar + 10% CW + 1.0 mg/l TDZ + 0.5 mg/l αNAA. After 4 weeks of culture, in vitro shoots produced adventitious roots and grew best (length of 7.36 cm/shoot, 7.03 leaves/shoot and 11.17 roots/shoot) when being grown in SH medium supplemented with 3% saccharose + 0.8% agar + 10% CW + 0.5 mg/l IBA.