STUDY ON GENETIC TRANSFORMATION OF FIBER LENGTH GENE (EcHB1) INTO TOBACCO AND EVALUATION OF GENE EXPRESSION

Abstract

In this study, the authors have used tobacco as model plant for studying the expression of fiber length gene (EcHB1), and the results of this study are the fundamental basis for transfering genes into some important forest trees such as Eucalyptus and Acacia so far. The EcHB1 gene has been transferred into Tobacco by Agrobacterium tumefaciens strain GV3101 with binary vector pGWB2/35S/EcHB1/NOS. Sixty-nine Tobacco transgenic lines have been produced, and PCR tests for 8 randomized transgenic lines have shown positive. After three months in nursery, thirty-two transgenic lines have reached the average height of 31.88 cm, while the control is only 17 cm in height. The fiber length of transgenic clones is also 12% higher than that of the control.