Developement of a LAMP (Loop-mediated isothermal amplication) assay for detection of Fasciola spp

Abstract

Fasciola spp. have two species, Fasciola gigantica
and Fasciola hepatica, which cause disease mainly in
herbivores such as buffaloes, cows, sheep, etc. and cause
disease in humans. According to the World Health
Organization (WHO), the disease is currently circulating
in 75 countries and territories around the globe. WHO
considers the disease a health issue that needs attention
in public health programs and has been ranked as an
important position in health strategy and policy by many
countries. Therefore, developing a fast and accurate
technique to detect Fasciola infection is very important
and necessary. In this study, the authors developed the
LAMP technique to detect Fasciola spp. from different
kinds of sample such as feces, tissue samples, etc. The
advantages of this method included high specificity,
fast detection time, and simple equipment use. Primer
set was designed for founding on ITS2 gene. A positive
reaction was visualised with the naked eye, using the
color indicator, malachite green.