In vitro evaluation α-glucosidase inhibitory, flammatory and antibacteria vities of selenium enriched

Abstract

Ophiocordyceps sinensis (O. sinensis) - commonly
known as Dong Chong Xia Cao, is a valuable medicinal
fungus containing many bioactive compounds such as
cordycepin, D-mannitol, polysaccharide, etc. While
selenium is an essential trace element for humans, it is
the main component of some enzymes such as glutathione
peroxidase, thioredoxin reductase, and deiodinase which
play an important role in scavenging free radicals,
enhancing reproductive and muscle function, and
preventing cancer. Several studies have shown that
inorganic selenium added to O. sinensis culture can be
converted into a beneficial organic form, which increases
the value for the fungus. In this study, extracts from
Se-enriched O. sinensis were evaluated some biological
activities such as anti-inflammatory, antibacterial,
and α-glucosidase inhibition. Results showed that Bu
extract (extracted by n-butanol), crude extract, and
EtOAc extract (extracted by ethyl acetate) can resist
to S. sonnei at a concentration of 1000 µg/ml. Besides,
the anti-inflammatory activity was stronger in crude
extract, PE extract, and EtOAc extracts with IC50 values
were 195.58±7.89; 64.19±0.22; 469.27±16.57 (µg/ml),
respectively. However, all extracts had no ability to
inhibit α-glucosidase. This study exhibited that extracts
from Se-enriched O. sinensis have an admirable
anti-inflammatory activity, which is a prerequisite for in
vivo model research.