Expression of TIF-IA protein and biosynthesis of ribosomal RNA highly inscrease in colorectal cancer patients’ tumors

Abstract

Colorectal cancer is amongst the five most common cancers worldwide, and has the second highest fatality rate behind lung cancer. The signs and symptoms of this disease are often difficult to detect in early stages. The current treatment methods generally are surgery, chemotherapy, radiation therapy, and targeted biodrugs. However, many patients suffer the relapse after treatment. A better understanding of the cellular and molecular mechanisms of colorectal cancer is required for developing more effective therapies. In this study, the authors investigated the expression of TIF-IA protein in colorectal cancer tissues in comparison with adjacent non-neoplastic tissues and also evaluated its effect in regulation of rRNA biosynthesis by real-time quantitive PCR (qRT-PCR) and Western-blotting analysis. Results showed that the expression of TIF-IA in colorectal cancer tissue samples was significantly higher than in the adjacent non-neoplastic samples. Moreover, the expression levels of TIF-IA was highly correlated to the amount of rRNA synthesized. These results suggest that TIF-IA plays an important role in the activities of colorectal cancer cells.