Formulation of throat spray emulsion containing eucalyptol

Abstract

The aim is formulation of throat spray emulsion containing eucalyptol which prevents and supports the treatment of pharyngitis and diseases related to upper respiratory tract infection. Methodology: an I-Optimal Experimental Model was designed by Design-Expert software v.8.0.6 including 21 formulas. Independent variables were percentage of eucalyptol (%), cremophor RH40 (%), ethanol (%), and poloxamer 407 (%). Dependent variables were levels of separating emulsion and average diameter of spray pattern. Based on ANOVA results and the influence of independent variables on dependent variables, Design-Expert software offered optimal formulas with different desirabilities. Final formulas were evaluated in vivo anti-inflammatory activity and such criteria as: perception, pH, density, spray pattern, delivered-dose uniformity, and determination of eucalyptol compound. The results were as follows: an experimental model with 21 formulas was established. Through the analysis of experimental data, Design-Expert software proposed optimal formulas with different desirabilities. The highest desirability formulas with 5 and 8% eucalyptol were selected; the anti-inflammatory activity was good when compared with other products on the market. The finished product was translucent, fragrant, lightly sweet and bitter; pH ranged between 4.5 and 6.5; density ranged from 0.98 to 1.01; spray pattern was round or almost round with the diameter of 6.8-9.2 cm; delivered-dose uniformity target was satisfied; and there was the appearance of blue to purplish red of eucalyptol on the chromatogram. In Conclusion, the two optimal formulas of eucalyptol throat spray emulsion met the quality requirements of preparation and also exhibitedthe anti-inflammatory activities.