Study on determining some nutritional components and optimal conditions for the ultrasonic extraction of saponin triterpenoid and total phenolic content from Ganoderma atrum in Nghe An

Abstract

The objective of this study was to investigate the nutritional composition and the optimal conditions of ultrasonic extraction of total saponin triterpenoid (TST) and total phenolic content (TPC) from Ganoderma atrum using response surface methodology (RSM). An optimal model according to the Box - Behnken design has been formulated to extract TST Y₁ and TPC Y₂ with three factors: extraction temperature (X₁), ultrasonic time (X₂) and ultrasonic power (X₃). Following this model, the optimal parameters for the extraction process to obtain the highest content of TST and TPC were extraction temperature of 65^oC, time of 83 mins and ultrasonic power of 310 W. The experimental values of TST and TPC were 1.08±0.04% and 122.52±0.15 mg GAE/g, respectively.