Isolation and selection of Streptomyces sp. species and Trichoderma sp. species against coffee berry disease by Colletotrichum sp. CC1.5 in Dak Nong province

Abstract

Anthracnose is one of the serious diseases on coffee trees. Prevention of coffee berry disease on coffee trees by biological measures is an alternative solution to the use of chemical pesticides. The study aims to select strains of Streptomyces and Trichoderma with strong antagonistic ability against Colletotrichum sp. CC1.5 fungus causing coffee berry disease. Results revealed that an actinomycete strain DR92S showed a high ability to antagonize Colletotrichum sp. CC1.5 after 8 days by direct antagonism and diffusion through agar holes, with the antagonism efficiency and inhibition zone of 71.85% and 28.63 mm, respectively. A strain of Trichoderma DR10T8 showed 100% inhibitory effects against Colletotrichum sp. CC1.5 after 5 days of treatment. The gene sequence analyses exhibited that the closest genetic relationships of the actinomycete strain DR9S2 and the fungal strain DR10T8 were Streptomyces hiroshimensis and Trichoderma viride, respectively.