Genotype identification and genetic diversity of the ompA gene among Chlamydia trachomatis isolated from infertile Vietnamese women

Abstract

This study aimed to identify the distribution and analysis of genetic diversity of Chlamydia trachomatis genotypes in infertile Vietnamese women. A total of 119 endocervical swabs were isolated from infertile women at the National Hospital of Obstetrics and Gynecology, Vietnam, during the period from January 2020 to December 2021, and were diagnosed with Chlamydia trachomatis infection. 81 sequences of the ompA gene
with a size of approximately 1,100 bp were used to determine the Chlamydia trachomatis genotypes by the current reference sequences from GenBank. Sequence analysis of the ompA gene from the 81 positive patients
showed nine genotypes, including E, D/Da, F, G/Ga, J, H, K, B/Ba, and I/Ia. In which, genotypes E, D/Da, and F accounted for 25.93, 22.22, and 13.58% respectively. Genotypes E, F, and G/Ga were highly conserved, showing 100% similarity to the alternative reference sequences, whereas ompA sequences of genotypes B/Ba, D/Da, H, I/Ia, J, and K displayed from one to ten nucleotide substitutions. In total, nineteen nucleotide changes were detected, seven of which led to amino-acid substitutions. The findings of the current study showed high prevalence of the genotypes E, D/Da, and F of C.trachomatis in infertile Vietnamese women.