Analysis of the mitochondrial genome and phylogenetic relationships of Mong chicken

Abstract

In this study, the complete nucleotide sequence of the mitochondrial genome of the Mong chicken and an indigenous chicken breed of Vietnam, was determined by the Sanger sequencing method, structurally analysed and used to evaluate the phylogenetic relationship with different chicken breeds. The complete nucleotide sequence data of the Mong chicken’ mitochondrial genome was registered on GenBank - National Center for Biotechnology Information (NCBI) with the accession number OR643716. The Mong chicken’ mitochondrial genome has a size of 16785 base pair (bp) and includes twenty-two transfer RNA genes, two ribosomal RNA genes, thirteen protein-coding genes, and one noncoding control region (D-loop). A, T, G, and C nucleotide proportions were 30.28, 23.76, 13.48, and 32.48%, respectively. Results of the analysis of phylogenetic relationships based on 33 complete mitochondrial genome sequences showed that the Mong chicken has evolutional origin from the red junglefowl subspecies Gallus gallus spadiceus. The Mong chicken is in close relationship with the Dong Tao chicken and Cao Son dwarf chicken in Vietnam, Rugao yellow chicken and Xiaoxiang chicken in China.