Research on the in vitro propagation of Dendrobium moschatum Buch.-Ham. ex Sw.

Abstract

To determine the optimal culture conditions for the in vitro propagation of Dendrobium moschatum Buch.-Ham. ex Sw., a rare orchid species facing a high risk of extinction, the study evaluated the effects of plant growth regulators (GA3 , BAP, TDZ, NAA) and activated charcoal on key developmental stages, including seed germination, shoot regeneration, and root induction prior to plantlet formation. All experiments were conducted under laboratory conditions. The results showed that Murashige & Skoog (MS) medium, supplemented with 3% sucrose and 5 g/l agar and adjusted to pH 5.8±0.1, produced the highest germination rate (89.67%). During the shoot regeneration stage, 3.0 mg/l BAP significantly promoted shoot formation, producing 4.25 shoots per explant. During the rooting stage, medium supplemented with 0.2 mg/l NAA and 2.0 g/l activated charcoal resulted in the highest rooting efficiency. The findings contribute to the optimisation of the in vitro propagation protocol for Dendrobium moschatum and support the conservation and development of this valuable orchid genetic resource.