An initial observation on the characteristics of cryopreserved tracheal allografts tissues at the Tissue Bank of Viet Duc University Hospital

Abstract

Data on tracheal preservation and post-preservation evaluation remain limited in the current medical literature in Vietnam. This study aims to describe the characteristics of tracheal tissues stored under in deep cryopreservation conditions at the Tissue Bank. Subjects and methods: Descriptive, prospective study on 13 tracheal tissue samples from brain-dead donors preserved at the Tissue Bank of Viet Duc Hospital. Results showed that 13 tracheal samples were collected from 13 brain-dead donors, with an average age of 35.2±15.2, including 9 males (69.2%) and 4 females (30.8%). Serological tests were negative for HIV 13/13 (100%), HCV 13/13 (100%), and HBV 12/13 (92.4%). During the recovery phase, the infection rate of tracheal tissue was 13/13 (100%). After decontamination, the negative culture rate was 9/13 (69.2%). The most common bacteria were Acinetobacter baumannii (8/18 strains) and Klebsiella pneumoniae (6/18 strains). 13/13 (100%) tracheal specimens retained their gross and microscopic structures after thawing for a storage period of 1-12 months. The cryopreserved tracheal tissue was successfully transplanted into a patient with post-traumatic laryngotracheal stenosis, showing positive outcomes within 1-3 months post transplantation.