Isolation and molecular identification of polyhydroxybutyrate-producing bacterial strain in Ho Chi Minh city

Abstract

Production of biopolymer is one of the topics attracting a great deal of interest in the field of biotechnology; it is considered having significant applications in our daily life. Various microorganisms, including wild types and recombinants have been selected due to their capability to produce Polyhydroxybutyrate (PHB). In this study, the authors attempted to isolate and select several bacterial strains which can biosynthesize PHB by Sudan Black B stain and identified the potential ones by 16S rRNA. Six bacterial strains were identified and classified into two groups: Enterobacter (Pseudomonas stutzeri strain H6, Enterobacter sp. H12, Klebsiella sp H14, Enterobacter sp. H56, Enterobacter cloacae strain H62) and Bacillus (Bacillus sp. H37). Two of them can be identified to the species, while the remaining ones can only be identified to the genera.