A study on the bud regeneration ability of Cymbidium wenshanense by in vitro culture

Abstract

The study on the buds regeneration of Cymbidium wenshanense orchid seedlings cultured in vitro in the conditions of 25^oC room temperature, 65% humidity, 2000 lux light intensity, and 16 hours of lighting a day was implemented with the aim of finding out the proper medium, cytokinin growth stimulators (kinetin, TDZ, and BA), and sucrose concentrations on seed regeneration. Results showed that MS medium had a good effect on seed germination (the highest is 90.54%) and cytokinin BA significantly improved the buds regeneration of Cymbidium wenshanense orchid (4.42 buds of 3.63 cm length, and 272.67 mg weight formed from a sample when 1 mg of BA was added). In addition, sucrose supplemented at 5% concentration was considered to be successful (4.47 buds of 3.6 cm length and 315 mg were produced when 5% sucrose was added). This is a result of practical significance in the conservation and development of Cymbidium wenshanense orchid in north Vietnam.