Sex determination by molecular markers and micropropagation of Actinidia chinensis

Authors

  • Thi My Hanh Nguyen
  • Thanh Tung Hoang, Dac Khai Hoang, Manh Cuong Do, Thi Nhu Mai Nguyen, Quoc Luan Vu
  • Van Biet Huynh
  • Huu Duc Huynh
  • Thi Nhu Phuong Hoang
  • Van Le Bui
  • Tan Nhut Duong*

Keywords:

Actinidia chinensis, sex, shoot cluster proliferation, silver nanoparticles

Abstract

In this study, a molecular sex-linked marker was used to determine the sex of 8 kiwifruit plants. In addition, leaf explants with sex determination were used as the original plant material for micropropagation via callus induction, shoot regeneration, and shoot cluster proliferation. The results showed that the sex determination method of Kiwi plants based on molecular markers on the Y chromosome amplified by primer pair SmY1_F/R successfully identified male Kiwi explant belonging to Actinidia chinensis. In addition, optimal explant surface sterilisation and shoot regeneration were obtained from leaf explants disinfected with 200 ppm silver nanoparticles; meanwhile, the culture medium supplemented with 20% coconut water was suitable for the highest efficiency of shoot cluster proliferation.

DOI:

https://doi.org/10.31276/VJST.64(7).54-59

Classification number

4.6

Author Biographies

Thi My Hanh Nguyen

Tay Nguyen Institute for Scientific and Research, VAST, 116 Xo Viet Nghe Tinh Street, Ward 7, Da Lat City, Lam Dong Province, Vietnam

Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet Street, Nghia Do Ward, Cau Giay District, Hanoi, Vietnam

Dalat Vocational Training College, 109 Yersin Street, Ward 10, Da Lat City, Lam Dong Province, Vietnam

Thanh Tung Hoang, Dac Khai Hoang, Manh Cuong Do, Thi Nhu Mai Nguyen, Quoc Luan Vu

Tay Nguyen Institute for Scientific and Research, VAST, 116 Xo Viet Nghe Tinh Street, Ward 7, Da Lat City, Lam Dong Province, Vietnam

Van Biet Huynh

Nong Lam University, Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Huu Duc Huynh

Biotechnology Center of Ho Chi Minh City, 2374 National Highway 1, Quarter 2, Trung My Tay Ward, District 12, Ho Chi Minh City, Vietnam

Thi Nhu Phuong Hoang

Dalat University, 1 Phu Dong Thien Vuong Street, Ward 8, Da Lat City, Vietnam

Van Le Bui

University of Science, Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam

Tan Nhut Duong*

Tay Nguyen Institute for Scientific and Research, VAST, 116 Xo Viet Nghe Tinh Street, Ward 7, Da Lat City, Lam Dong Province, Vietnam

Downloads

Published

2022-07-25

Received 25 February 2022; accepted 31 March 2022

How to Cite

Nguyen Thi My Hanh, Hoang Thanh Tung, Hoang Dac Khai, Do Manh Cuong, Nguyen Thi Nhu Mai, Vu Quoc Luan, Huynh Van Biet, Huynh Huu Duc, Hoang Thi Nhu Phuong, Bui Van Le, & Duong Tan Nhut*. (2022). Sex determination by molecular markers and micropropagation of Actinidia chinensis. Version B of Vietnam Journal of Science and Technology, 64(7). https://doi.org/10.31276/VJST.64(7).54-59

Issue

Vol. 64 No. 7 (2022): Volume 64 - Number 7 - July 2022

Section

Agricultural Sciences

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