Effect of cobalt, copper and molybdenum trioxide nanoparticles on in vitro rooting stage efficiency of strawberry (Fragaria x ananassaDuch.)

Abstract

This study has demonstrated the positive effects of CoNPs, CuNPs and MoO₃NPs on the in vitro rooting ability of strawberry (Fragaria × ananassa Duch.). The results indicated that strawberry plants cultured on Murashige and Skoog (MS) medium, in which COCl₂.6H₂O was replaced with 6.2 μl/l CoNPs (1:1 ratio), resulted in the best growth indicators of plant height, number of leaves, number of roots, root length, fresh weight, dry weight and soil plant analysis development (SPAD) after 30 days of culture (4.80 cm; 13 leaves/plantlet; 15.67 roots/plantlet; 1.97 cm; 248.33 mg; 25.56 mg; 48.70; respectively), which were higher than those observed in plants cultured on the basal MS medium (without CoNPs) and the other treatments. In addition, MS medium supplemented with 6.4 µl/l CuNPs replacing CuSO₄.5H₂O salt (ratio 1:1) gave optimal results in terms of number of leaves (15 leaves/plantlet), number of roots (16.67 roots/plantlet), root length (2.5 cm), fresh weight (224.60 mg), dry weight (22.86 mg) compared to the remaining treatments after 30 days of culture. These findings are promising and open up exciting possibilities for further research and advancements in Strawberry micropropagation.